Arden Bio

Profiling

The Deep Immune Profile

We coordinate blood draws at multiple timepoints and running a set of advanced assays to build a mechanistic picture of a patient’s immune state.

Immune Cell Profiling

High-dimensional flow cytometry, single-cell RNA sequencing, and single-cell ATAC-seq. T-cell subset skewing, monocyte activation states, NK cell phenotypes, regulatory deficits, and exhaustion signatures.

Blood Protein & Signaling

Plasma proteomics (thousands of proteins), clinically validated cytokine panel, type I interferon signature, complement pathway evaluation, and proteome-wide autoantibody discovery.

Genomic & Repertoire

Whole genome sequencing and T-cell/B-cell repertoire sequencing. Inborn errors of immunity, pharmacogenomic variants, and clonal expansions suggesting antigen-driven autoimmune reactivity.

Metabolic, Tissue & Microbiome

Plasma metabolomics, mitochondrial function testing, cell-free DNA methylation sequencing, and microbiome shotgun sequencing. Bioenergetic reserve, tissue-of-origin injury signals, and microbiome composition.

Not every patient requires every assay; the profile is tailored to answer the specific questions each case raises.

Framework

Five Immune Circuit Families

We organize thousands of data points into five immune circuits. Each one represents a different way your immune system can malfunction. This is what makes it possible to see why two patients with the same diagnosis can need very different treatments.

Circuit A

Innate-Inflammation

Your immune system's first responders (monocytes, macrophages) are stuck in overdrive. Instead of calming down after a threat, they keep causing tissue damage through persistent inflammatory signaling.

Circuit B

IFN / T Cell-Driven

Your interferon- or T cell-driven immune response is misdirected, attacking your own tissues. This is the pattern behind many classic autoimmune conditions where the immune system loses the ability to tell self from threat.

Circuit C

Humoral / Immune-Complex

Your B cells are producing antibodies that target your own body. These antibodies form complexes that deposit in tissues, triggering inflammation and organ damage.

Circuit D

Regulatory / Repair

Your immune system's "brakes" (regulatory T cells, gut microbiome signals, tissue repair pathways) aren't working properly. Without them, inflammation continues even after the original trigger is gone.

Circuit E

Effector-Stress

Your immune system's fighter cells are burned out or misfiring, often because of lingering viral infections, overactive mast cells, or clotting abnormalities tied to immune dysfunction.

Interpretive Overlays

Four Overlays That Modify Circuit Behavior

Overlays are background factors that change how circuits behave and respond to treatment. Two patients with the same dominant circuit can have very different outcomes depending on which overlays are present.

Inherited

Genetic variants you were born with that permanently shape how your immune system behaves. These can predispose you to specific circuit patterns and limit which therapies will work.

Immunosenescence

As you age, your immune system changes. It produces fewer new immune cells, relies more on worn-out ones, and runs a higher baseline of inflammation. This shifts which circuits dominate and how well treatments work.

Immunometabolic

Your immune cells need energy to function properly. When their internal power supply (mitochondria) is compromised, the immune system loses its ability to regulate itself, even if the right drugs are on board.

Tissue Injury

When organs are injured, they release DNA fragments into the blood. These fragments can be analyzed to help physicians identify which specific tissues are being damaged, often before standard tests would detect it.

Patient Summary Concept

Each patient’s immune dysregulation is summarized as a relative circuit-and-overlay mix. The summary maps directly to treatment options.

"A high, B moderate, with Z3 metabolic constraint and Z4 epithelial damage"

Application

From Profile to Strategy

The profiling readout maps directly to rule-in / rule-out therapeutic decisions. Each circuit and overlay points to a specific class of interventions your physician can evaluate.

Circuit / Overlay	Potential Interventions to Consider (Examples)
A – Innate	IL-1/IL-6/TNF inhibitors; colchicine; NLRP3 inhibitors
B – IFN / T cell	Anifrolumab; JAK inhibitors; anti-IL-12/23; anti-IL-17
C – Humoral	Rituximab; BAFF/complement-targeted agents; plasmapheresis
D – Regulatory	Low-dose IL-2; microbiome-directed Treg induction
E – Effector-stress	Mast-cell stabilizers; antivirals; complement inhibitors
Z1 – Genetic	Variant-specific targeted therapy
Z3 – Metabolic	mTOR modulators; NAD+ precursors; CoQ10 adjuncts
Z4 – Tissue	Organ-protective strategies; cfDNA monitoring

Physician authority. All therapeutic considerations are presented for physician evaluation. Arden provides investigative depth and treatment-relevant analyses. Your physician retains full clinical authority over treatment decisions.

Each engagement runs in analytical cycles: measure, synthesize, inform. Your care team adjusts based on real data, not guesswork. And because each cycle is structured like a protocol, you can actually tell whether something worked or didn’t.

From over 10,000 data points to mechanism-level clarity.